Kirby Bauer

Lab Report 3: Antibiotic Evaluation by the Kirby-Bauer Method Introduction Chemical antimicrobial agents atomic soma 18 chemical compounds capable of either inhibiting the reaping of microorganisms or sidesplitting them outright. Those which are taken internally to alleviate the symptoms of or promote healing from disease are called chemotherapeutic do drugss, and among these is a class of compounds called antibiotic drugs. In order for a chemotherapeutic drug to be classed as an antibiotic, it mustiness be produced by a microorganism such as bacteria or fungus or at least derived from a chemical produced by one. It must also be capable of killing or inhibiting the growth of other microorganisms and of doing so when taken in very clarified quantities. To study whether a microbial harvest-feast qualifies as an effective antibiotic, a ensample procedure called the Kirby-Bauer manner is employed. This method, which is the procedure recommended by the US nutri ment and Drug Administration, was devised by William Kirby and A.W. Bauer in 1966. In the current protocols touch on in the Kirby-Bauer method, Mueller-Hinton standard agar is used as the culture medium for bacterial culture. The pH of the standard agar is 7.2 to 7.4 and it is poured exclusively to a depth of 4 mm.

The medium is heavily inoculated with bacteria and piece dish antennas containing enough of the antibiotic under study to create an optic density of 1 (the McFarland standard) are placed on outmatch of the cultures. By examining the results of incubation in the form of a govern of ban more or less each disk after incubation, it can be ascertain how effect! ive each antibiotic is against any given bacterium. A minimum restrictive concentration can then be deduced for the given antibiotic vs. the specific bacterium tested so that appropriate loony toons may be determined. Resistant bacteria cultures will ground a small or no zone of inhibition if their growth is not sufficiently control for the antibiotic to be a viable candidate in treating infection by that...If you want to get a affluent essay, order it on our website: BestEssayCheap.com

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